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Description
Alexa Fluor® 647 Rat Anti-Mouse CD34 Antibody (S-R599)Product Specification Host Rat Antigen CD34 Synonyms Hematopoietic progenitor cell antigen CD34 Location Membrane Accession Q64314 Clone Number S R599 Antibody Type Rat mAb Isotype IgG2a,k Application FCM Reactivity Ms Purification Protein G Concentration 0. 2 mg ml Conjugation Alexa Fluor 647 Physical Appearance Liquid Storage Buffer PBS, 1% BSA, 0. 3% Proclin 300 Stability & Storage 12 months from date of receipt reconstitution, 2 to 8 C as supplied
Product Specification
| Host | Rat |
| Antigen | CD34 |
| Synonyms | Hematopoietic progenitor cell antigen CD34 |
| Location | Membrane |
| Accession | Q64314 |
| Clone Number | S-R599 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
CD34 is a transmembrane phosphoglycoprotein first identified on hematopoietic stem and progenitor cells. It is a member of the sialomucin family, with other members including podocalyxin and endoglycan. This protein functions primarily as an adhesion molecule, involved in cell-cell adhesion and cell-matrix interactions. It enhances the proliferation of progenitor cells and blocks cell differentiation, which is crucial for maintaining a pool of immature cells that can develop into various specialized cells as needed. CD34 also plays a role in immune system regulation, particularly in the adhesion of lymphocytes to blood vessel walls. Besides hematopoietic cells, CD34 is expressed on endothelial cells, fibrocytes, muscle satellite cells, and some cancer stem cells. Its expression has been utilized for the selection and enrichment of hematopoietic stem cells for bone marrow transplants.
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