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Description
Alexa Fluor® 647 Mouse Anti-Human DAF/CD55 Antibody (S-628-48)Product Specification Host Mouse Antigen DAF CD55 Synonyms Complement decay accelerating factor; CR Immunogen Recombinant Protein Location Cell membrane Accession P08174 Clone Number S 628 48 Antibody Type Mouse mAb Isotype IgG1,k Application FCM Reactivity Hu Purification Protein G Conjugation Alexa Fluor 647 Physical Appearance Liquid Storage Buffer PBS, 25% Glycerol, 1% BSA, 0. 3% Proclin 300 Stability & Storage 12 months from date of receipt
Product Specification
| Host | Mouse |
| Antigen | DAF/CD55 |
| Synonyms | Complement decay-accelerating factor; CR |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P08174 |
| Clone Number | S-628-48 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Purification | Protein G |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5 μl per million cells in 100μl volume |
Background
Decay-accelerating factor (DAF, also known as CD55) is a complement regulatory protein that plays a crucial role in protecting cells from complement-mediated attack. It is a membrane lipid microdomain-associated, GPI-anchored protein that accelerates the decay of C3 and C5 convertases, which are essential for the downstream formation of the membrane attack complex (MAC) in the complement cascade. This action helps prevent excessive complement activation and subsequent cell damage. CD55 is involved in various physiological and pathological processes, including immune regulation, cancer progression, and tissue protection. In cancer, CD55 can promote malignant transformation, cell survival, angiogenesis, and inhibit apoptosis through intracellular signaling pathways such as JNK, JAK/STAT, MAPK/NF-κB, and LCK. Additionally, it is enriched in the cancer stem cell niche and can be induced by chemotherapeutics and hypoxic environments.
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4.3 ★★★★★
Based on 1815 reviews
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Product Reviews
★★★★★ 5
Gentle on highly sensitive skin
Style: Updated Formula
I have spent so long looking for a product that is not only hydrating but calming enough for my Rosacea. This is it!! buy 5 of them because my skin has never been so soft and looks great!
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Reviewed in the United States on February 2, 2026
★★★★★ 5
Well made
I like that it’s lightweight very vintage looking
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Reviewed in the United States on May 17, 2026
★★★★★ 5
Obsessed. Soothing, effective, fragrance-free, incredibly healing
Style: Tolerance Daily Foaming Facial Cleanser
I’ve been loving this so much I bought a second bottle after a few days of using it. It has all the skin soothing benefits of the tolerance lotion cleanser, but it’s also a really great cleanser. It’s almost not noticeable how good it is at cleansing because your skin doesn’t feel stripped after, but it takes things off really quickly. I was worried because it doesn’t foam a lot and it feels so nurturing — much like the tolerance lotion cleanser (which I use as a second cleanse when my skin needs a few days to recover from something like a derm office treatment) — but when I wash again with my favorite HG cleanser nothing extra comes off, and I tested it twice on waterproof eyeliner that I’d swiped on my forearm for an hour or so, the eyeliner dissolved and wiped off with two or three gentle swipes. It has a very emollient texture, so when you rub it against your skin it slips so easily you don’t realize it’s dissolving and taking things away because there is no friction. So it’s great at taking off makeup, it’s AMAZING at cleaning makeup brushes, and IT DOES NOT BURN MY EYES AT ALL. Also, it is FRAGRANCE-FREE… before I bought it I read a review complaining about smelling a citrus fragrance, that person either has some sort of brain tumor or something spilled on their bottle when it was in a warehouse because this is definitely a fragrance-free product.
Prior to trying this, I had tried another cleanser that gave me weird acne hives all over my chest (never happened to me before) and I was struggling to heal the acne and the irritation. I noticed an immediate difference when I started using the tolerance cleanser on it; the fresh redness went away, the acne started healing, and the post-inflammatory hyperpigmentation has been healing fast. So I’ve been using this on my body as well and it’s soothing and moisturizing in all the places where other things have been drying. I have mild rosacea and I hadn’t realized how irritated my skin was from the last thing I’d tried until after 5 days or so of using this my skin tone looked much more even and the stubborn PIH I have from a two-zit hormonal breakout a month ago finally looked dramatically lighter (as recently as the week before, I’d needed to cover them with high-coverage concealer).
Because this doesn’t foam much, some people might not realize how well it’s cleaning… I encourage you to judge it based on how your skin looks and feels. Since it is very emollient though, I do think it’s important to use it with a face wash scrubber or washcloth just to ensure you’re getting some mild mechanical exfoliation and clearing away skin cells that could be trapping stuff in your pores. For this reason, I do wash my face with this twice so the first wash can get off makeup/debris/oil and then the second wash can really penetrate and clean my skin.
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Reviewed in the United States on February 15, 2026
★★★★★ 5
No Scent and Gentle
Style: Tolerance Daily Foaming Facial Cleanser
I broke out in a case of Perioral Dermatitis recently and bought this product to help before I could see my doctor. Avene Tolerance facial cleanser has no scent and is very gentle on my skin. It didn’t burn when washing with this product, and even helped to slough off the dead skin around my lips. My face feels clean, not dry. I am still going to see my doc to get an RX for the inflammation, but I do believe this product helped to alleviate the dryness and tightness of my skin and lips. I’ve used other Avene products and I’m very happy with them. Even though these products are more expensive, to me they are well worth the money because they are much more gentle on the skin than any other products I’ve used. I will buy this product again just because of the fact that it’s so gentle. PS: at the time of purchase for this product, I also bought Avene’s Tolerance Control Skin Recovery Cream (you don’t need to use a lot of this one on your entire face), and Avene’s Cicalfate for lips. Between these 3 products, my lips and skin feel a lot better (however, I will still see my doctor because I have Crohn’s Disease and Perioral Dermatitis is considered an extra-intestinal side effect of Crohn’s).
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Reviewed in the United States on March 20, 2026
★★★★★ 5
Gentle everyday cleanser!
Style: Tolerance Daily Foaming Facial Cleanser
My go to nightly cleanser! Gentle yet effective. Removes makeup without leaving any residue behind. Also does not dry out my skin or cause breakouts! I would say it’s more of a cream cleanser though. It does not really foam up. It’s a decent size bottle for the price. The pump dispenser is an added plus!
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Reviewed in the United States on May 19, 2026
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