Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 31 - Aug 5
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human CRYM ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Crystallin mu (CRYM) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Crystallin mu (CRYM) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Crystallin mu ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Thiomorpholine carboxylate dehydrogenase (Mu-crystallin homolog), also known as NADP-regulated thyroid hormone-binding protein (THBP), is a protein encoded by the CRYM gene. Multiple alternatively spliced transcript variants have been identified for this gene. Crystallins are divided into two categories: taxon-specific and ubiquitous. The former are also known as phylogenetically restricted crystallins. The latter constitute the primary protein of the vertebrate eye lens, maintaining its transparency and refractive index. This gene encodes a taxon-specific crystallin that binds NADPH and shares sequence similarity with bacterial ornithine cycloaminase. The encoded protein does not play a structural role in lens tissue, but rather binds thyroid hormone, potentially playing a regulatory or developmental role. The enzymatic function has been identified as a ketimine reductase, reducing cyclic ketimines to their reduced form. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.5 ★★★★★
Based on 7 reviews
Sort
Product Reviews
★★★★★ 5
Open our eyes
Format: Paperback
I recently finished reading Joash's very insightful, inspirational, and encouraging book. I hope it is read and implemented far and wide. His interpretation of Jesus and "the church" is as close to the spirit of Scripture as I can find. Twenty years ago, I read "Jesus and the Disinherited" by Howard Thurman that led me to places and situations and cultures and events which were not part of my own life's context. It was Jesus' context and I began to understand his lessons through his eyes instead of my own. Joash, like Howard Thurman, is right - we colonized, Caucasian, Western civilization folks just don't get it. But we should and we need to. Open your mind to read this book and see things through God's eyes. Thank you Joash!! We should all look for ways to extend this teaching to others. "...thy will be done, on Earth [all of earth and humanity] as it is [and will be] in Heaven.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 6, 2025
★★★★★ 5
A Bold, Pastoral Call to Decolonize the Gospel
Format: Paperback, Format: Paperback
When I began my journey of theological deconstruction, I quickly realized that shedding the harmful elements of conservative white evangelicalism wasn’t enough. The deeper I went, the more I saw how white Christianity remains entangled with colonial narratives that uphold social and sexual hierarchies and distort the liberating truth of the Gospel.
Rev. Joash Thomas’s *The Justice of Jesus* is the book I didn’t know I needed. His voice has profoundly shaped my theological imagination, and this work is a masterclass in liberation ecclesial theology. With clarity and courage, he names the perversion of white, colonialist, slaveholder theology and invites readers to confront its lingering presence in our churches, our pulpits, and our lives.
What sets this book apart is its balance of prophetic fire and pastoral tenderness. Rev. Joash never veers into cynicism or outrage for outrage’s sake. Instead, he offers practical, Spirit-led guidance for reclaiming the Gospel from the sin of colonialism and whiteness. He casts a vision of Eucharistic unity, a table wide enough to defy empire and deep enough to hold our collective liberation in Christ.
This book emboldened me to use my voice and privilege to pursue the decolonization of theological practice in my own context. I cannot recommend it highly enough to anyone seeking a Gospel that liberates, heals, and restores.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 3, 2025
★★★★★ 5
Un libro necesario para la iglesia hoy
Format: Paperback, Format: Paperback
As a believer from the Global South. As a child who first heard the liberating message of Jesus through integral mission in Latin America. Yet, over the years, I was also exposed to a highly colonized version of the gospel—one that increasingly abandoned the most vulnerable and aligned itself with a form of Christianity distant from real human needs. Reading this book restores my hope, not because change will come quickly or easily, but because it opens the space for the necessary conversations of reevaluation.
Joash, in a very direct way, lays out the causes and effects of inherently unjust colonizing systems that have broken entire societies, and shows how these systems have permeated the way we live out the gospel of Jesus. By bringing these to light in various areas, he challenges the Western church to reconsider its practices, to move alongside the oppressed, and to become aware of its own shortcomings. At the same time, he reminds us that hope remains, that we have much to learn from the Global South, and that there are many silenced voices that must urgently be heard.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 2, 2025
★★★★★ 5
Generous, inclusive, and deeply Christian.
Format: Paperback
What an amazing book this is! Joash Thomas challenges western Christians to sit in dialogue with global south brothers and sisters, and to be willing to learn from them. If justice means everyone having what God wants them to have, then why is justice not a priority for so many of us? Never scolding, and never despairing, Joash walks us through the lessons we could take away from such a meeting, and offers actual strategies for incorporating "justice work" into the lives of western churches. A MUST READ!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 3, 2025
★★★★★ 5
Bathroom complete
Color: Warm Gold
Perfect
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 4, 2026
recommand products
ARB Safari 4X4 Snorkel Rspec Suzuki Jimny 1.3L G13B 1/98-12 - SS820R
188.50
ARB Ss170Hf Boxed Fitting Kit - SS170HFBOXFK
161.00
ARB Snorkel Suits Jeep Jl Wrangler - SS1080HF
349.98
ARB Ss1050Hf Box Fit Kit - SS1050HFBOXFK
189.50
ARB Safari 4X4 Snorkel Vspec Tacoma Gen 1 V6 3.4L Pet 95-04 - SS170HF
251.50