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Description
Human MDM2 (E3 Ubiquitin-protein Ligase Mdm2) ELISA KitThe Human (MDM2) E3 Ubiquitin protein Ligase Mdm2 ELISA Kit measures E3 ubiquitin protein ligase Mdm2 in samples. The plate has been pre coated with Human MDM2 antibody. MDM2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human MDM2 Antibody is added and binds to MDM2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated MDM2 antibody. After incubation unbound Streptavidin HRP is
The Human (MDM2) E3 Ubiquitin-protein Ligase Mdm2 ELISA Kit measures E3 ubiquitin-protein ligase Mdm2 in samples. The plate has been pre-coated with Human MDM2 antibody. MDM2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human MDM2 Antibody is added and binds to MDM2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MDM2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human MDM2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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